Tissue Culture Unit

Personnel

Head of Unit

Ana Grenha

Tel: 289 244 443

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About

The CBMR Tissue Culture Unit (TCU) is constituted by a Biosafety Level 1 (BL1) and a Biosafety Level 2 (BL2) tissue culture rooms. This unit provides the required environment and equipment for a wide range of cell and tissue culture procedures, from maintenance and manipulation of cell lines and tissue samples, to cell observation and data analysis. In addition the unit provides routine mycoplasma detection testing for mammalian cell lines.

The TCU is equipped with 7 laminar flow hoods, 3 CO2 incubators and 2 CO2/O2 incubators that allow the regulation of oxygen levels for hypoxia experiments (0% to 21% O2), 3 inverted microscopes (one of which is a fluorescence microscope coupled to an imaging system, Axiovert 40 cfl Microscope) and accessory equipment (e.g. centrifuges, water bath, refrigerators, freezers).

 

HERA Safe class II laminar hood (Heraeus) Heraeus hood

Bio II Advance class II laminar hoods (Telstar) Telstar Hood

Advanced Bio Safety Cabinet-class II laminar hood (Microflow) Microflow hood

MSC-advantage 1.2 class II laminar hood (Thermo Scientific) MSC-advantage class II

Forma Series II Water Jacketed CO2/O2 Incubators CO2/ O2 incubator

Forma Steri-Cycle CO2 Incubators (Thermo Scientific) CO2 incubator

Axiovert 40 cfl Microscope (Carl Zeiss) Axiovert40

Major Equipment

Primary Culture Hood

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Viral Particle Hood

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General Use Hood

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