The Pharmacogenomics and Molecular Toxicology Laboratory

v_marques_01

Location

Building 8, Laboratory

Department of Biomedical Sciences and Medicine.

University of Algarve

Phone: +351 289 244 442

vmarques@ualg.pt

Team Members

Vera Marques (Principal Investigator)

Kamila Smieszkol (MSc Student)

Zhaneta Shala (MSc Student)

Diana Rosli (MSc Student)

About the PGMTL

The Pharmacogenomics Laboratory (PGMTL) aims at the identification of gene polymorphisms associated with individual risk, both for disease and toxicity or efficacy of a given treatment. Additionally, the molecular and structural determinants underlying the development of disease are studied within the PL at the level of transcriptional regulation of genes relevant for the clearance of chemicals, under the influence of external and internal stimuli. Molecular biology and pharmacogenomic approaches are used both in in vitro and in vivo disease models, as well as in population-based case-control and cohort studies, in the frame of national and international networks.

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CYP7A1, CYP27A1 and CYP7B1 polymorphisms. Patterns of DNA fragments obtained for each genotype identified. PCR-RFLP analysis was performed using specific primers and enzymes.

Publications (2008-2014)

Dias, V, Ribeiro, V. Ethnic differences in the prevalence of polymorphisms in CYP7A1, CYP7B1 and CYP27A1 enzymes involved in cholesterol metabolism. J Pharm Bioallied Sci. 2011, 3(3):453-9.

Teixeira VH, Ribeiro V, Martel PJ. (2010) Analysis of binding modes of ligands to multiple conformations of CYP3A4.Biochim Biophys Acta. 1804(10):2036-45.

Félix, RC, Müller, P, Ribeiro, V, Ranson, H, Silveira, H (2010) Plasmodium infection alters Anopheles gambiae detoxification gene expression. BMC Genomics 2010, 11:312

Butenko, N, Tomaz,AI, Nouri,O, Escribano,E, Moreno,V, Gama,S, Ribeiro,V, Telo,JP, Pesssoa,JC, Cavaco,I. DNA cleavage activity of (VO)-O-IV(acac)(2) and derivatives. J Inorg  Biochem 2009,  103: 622-632.

Veiga MI, Asimus S, Ferreira PE, Martins JP, Cavaco I, Ribeiro V, Hai TN, Petzold MG, Björkman A, Ashton M, Gil JP. Pharmacogenomics of CYP2A6, CYP2B6, CYP2C19, CYP2D6, CYP3A4, CYP3A5 and MDR1 in Vietnam. Eur J Clin Pharmacol. 2009, 65:  355-363.

Ribeiro, AL, Correia,J, Ribeiro, V. Ethnic variability of HIF-1α polymorphisms.  Cancer Biomarkers 2009, 5: 1–5. DOI 10.3233/CBM-2009-0112.

Cavaco, I, Piedade, R, Ribeiro, V. (2008) Chapter 5. Cytochrome P450 Pharmacogenetics in the Portuguese Population, in FARMACOGENÉTICA DE POBLACIONES IBEROAMERICANAS, pp.34-38 (ISBN pending).

Veiga MI, Asimus S, Ferreira PE, Martins JP, Cavaco I, Ribeiro V, Hai TN, Petzold MG, Björkman A, Ashton M, Gil JP. Pharmacogenomics of CYP2A6, CYP2B6, CYP2C19, CYP2D6, CYP3A4, CYP3A5 and MDR1 in Vietnam.  Eur J Clin Pharmacol. 2008 Nov 1. (DOI 10.1007/s00228-008-0573-8)

Dorado, P, Cavaco, I, Cáceres, M., Piedade, R; Ribeiro, V., Llerena, A. (2008) “Relationship between CYP2C8 genotypes and diclofenac 5-hydroxylation in healthy Spanish volunteers”, Eur J Clin Pharmacol., 64(10):967-70.

Ferreira PE, Veiga MI, Cavaco I, Martins JP, Andersson B, Mushin S, Ali AS, Ribeiro V, Björkman A, Gil JP. (2008) “Polymorphism of malaria drug metabolizing, nuclear receptor and drug transport genes in Zanzibar” Therapeutic Drug Monitoring, 30(1):10-15.

Project Grants

Project Leader

“Crosstalk between hypoxia signalling and environmental agents in cancer”, Fundação Gulbenkian de Ciência.

POCTI/CBO/48771/2002 – “ Adaptation to neonatal life -the liver transcriptome in the perinatal period”. Fundação para a Ciência e Tecnologia, Area Health Sciences.

POCTI/MGI/34699/99 – “ Isolation and characterization of Cytochrome P450 (CYP) genes in Plasmodum falciparum ”. Fundação para a Ciência e Tecnologia, Area Health Sciences.

Team Leader

206RT0290 – Red Iberoamericana de Farmacogenética: Impacto en salud publica. CYTED.

POCI/BIO/61912/2004 – “Piezoelectric and electrochemical DNA sensors for simultaneous DNA hybridization and polymorphism detection”, Fundação para a Ciência e a Tecnologia, Area Biotechnology.

POCI/QUI/63166/2004 – “Theoretical and experimental studies of enzyme conformation, energetics and dynamics of CYP3A4.” Fundação para a Ciência e a Tecnologia, Area Chemistry and Biochemistry.

POCTI/MGI/48417/2002 – “Relationship between CYP3A4 gene polymorphism, protein structure and activity in disease setting”, Fundação para a Ciência e a Tecnologia, Area Health Sciences.

POCTI/ESP/39580/01 – “Studies on the human host pharmacogenetics and the use of antimalarials” Fundação para a Ciência e Tecnologia, Area Health Sciences.

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