Building 8, Laboratory 1.19
Department of Biomedical Sciences and Medicine.
University of Algarve
Phone: +351 289 800 094
Inês Araújo , PhD (Principal Investigator)
Sónia Simão, PhD
Bruno Pereira Carreira, MD, PhD
Ana Isabel Santos, PhD
Ana Sofia Lourenço (PhD student)
Joana Silva (PhD student)
Vera Henriques (MSc Student)
Ana Mestre (MSc)
João Moreira (BSc)
About the NL
The Neurogenesis Laboratory (NL) is interested in the study of adult neurogenesis and brain repair. Using both in vitro and in vivo approaches, the NL group is investigating the mechanisms that regulate neural stem cell proliferation and differentiation with particular emphasis directed towards several disorders including neuro-inflammation conditions, such as epilepsy, stroke or neurodegenerative disorders. The NL unit is particularly interested in understanding the mechanisms of how post-injury neurogenesis occurs in the lesion micro-environment and in developing new ways to achieve efficient endogenous neurogenesis and/or successful survival and maturation of grafted new neurons.
Santos AI, Carreira BP, Izquierdo-Álvarez A, Ramos E, Lourenço AS, Santos D, Morte MI, Ribeiro LF, Marreiros A, Sánchez-López N, Marina A, Carvalho CM, Martínez-Ruiz A, Araújo IMCA (2017) S-nitrosylation of Ras mediates nitric oxide dependent post-injury neurogenesis in a seizure model. Antioxidants and Redox Signaling. 2017 Jun 24. doi: 10.1089/ars.2016.6858. (Epub ahead of print)
Machado VM, Lourenço AS, Florindo C, Fernandes R, Carvalho CM, Araújo IMCA (2017) Overexpression of calpastatin preserves cognitive function following seizures, while mantaining post-injury neurogenesis. Frontiers in Molecular Neuroscience 10:60.
Rochar PRF, Medeiros MCR, Kintzel U, Vogt J, Araújo IM, Mestre A, Mailänder V, Schlett P, Dröge M, Schneider L, Biscarini F, de Leeuw DM, Gomes HL (2016) Extracellular electrical recording of pH-triggered bursts in C6 glioma cell populations. Science Advances 2(12):e1600516.
Medeiros MCR, Mestre A, Inácio P, Asgarif S, Araújo IM, Hubbard PC, Velez Z, Cancela ML, Rocha PRF, de Leeuw DM, Biscarini F, Gomes HL (2016) An electrical method to measure low-frequency collective and synchronized cell activity using extracellular electrodes. Sensing and Biosensing Research 10:1-8. URL: http://www.sciencedirect.com/science/article/pii/S2214180416300654.
Machado NJ, Simões AP, Silva HB, Ardais AP, KAster MP, Garção P, Rodrigues DI, Pochmann D, Santos AI, Araújo IM, Porciúncula OL, Tomé AR, Kofalvi A, Vaugeois J-M, Agostinho P, El Yacoubi M, Cunha RA, Gomes CA (2016) Caffeine reverts memory but not mood impairment in bred-based depressed mice with up-regulated adenosine A2A receptor in hippocampal glutamate synapses. Molecular Neurobiology. URL: https://www.ncbi.nlm.nih.gov/pubmed/26860412.
Santos DM, Martins AR, Casanellas I, Ova AB, Araújo IM, Power D, Tiscornia G (2016) Ear wound regeneration in the African Spiny Mouse Acomys cahirinus. Regeneration 3(1):52-61.URL: https://www.ncbi.nlm.nih.gov/pubmed/27499879.
Carreira BP*, Santos DF*, Santos AI, Carvalho CM, Araújo IM CA (2015) Nitric oxide regulates neurogenesis in the hippocampus following seizures. Oxidative Medicine and Cellular Longevity 2015:451512. URL: https://www.ncbi.nlm.nih.gov/pubmed/26587180.
Carreira BP*, Santos DF*, Santos AI, Carvalho CM, Araújo IM CA (2015) Nitric oxide regulates neurogenesis in the hippocampus following seizures. (Accepted on May 18, 2015, in Oxidative Medicine and Cellular Longevity)
Santos AI, Martinez-Ruiz A, Araújo IM CA (2015) S-nitrosation and neuronal plasticity. British Journal of Pharmacology172(6): 1468-1478.
Machado VM, Morte MI, Carreira BP, Azevedo MM, Takano J, Iwata N, Saido TC, Asmussen H, Horwitz AR Carvalho CM,Araújo IM CA (2015) Involvement of calpains in adult neurogenesis: implications for stroke. Frontiers in Cellular Neuroscience 9:22.
Carreira BP, Morte MI, Santos AI, Lourenço AS, Ambrósio AF, Carvalho CM, Araújo IM CA (2014) Nitric oxide from inflammatory origin impairs neural stem cell proliferation by inhibiting epidermal growth factor receptor signaling. Frontiers in Cellular Neuroscience 8: 343.
Santos AI, Carreira BP, Nobre RJ, Carvalho CM, Araújo IM (2014) Stimulation of neural stem cell proliferation by inhibition of phosphodiesterase 5. Stem Cells International 2014:878397.
Carreira BP, Santos AI, Carvalho CM and Araújo IM (2013) Modulation of adult neurogenesis by the nitric oxide system in Neural Stem Cells. Edited by Intech. Editor: Luca Bonfati. ISBN:980-953-307-863-7
Morte MI, Carreira BP, Falcão MJ, Ambrósio AF, Soares-da-Silva P, Araújo IM, Carvalho CM, (2013) Evaluation of neurotoxic and neuroprotective pathways affected by antiepileptic drugs in cultured hippocampal neurons. Toxicology in vitro 27(8):2193-9202
Morte MI, Carreira BP, Machado VM, Carmo A, Nunes-Correia I, Carvalho CM, Araújo IM (2013) Evaluation of proliferation of neural stem cells in vitro and in vivo. Current Protocols in Stem Cell Biology 2013 Feb; Chapter 2:Unit2D.14.
Martinez-Ruiz A, Araújo IM, Izquierdo-Álvarez A, Hernansanz-Agustín P, Lamas S, Serrador JM (2013) Specificity in S-nitrosylation: a short-range mechanism for NO signaling? Antioxidants and Redox Signaling 19(11):1120-1135.
Carreira BP, Carvalho CM, Araújo IM (2012) Regulation of injury-induced neurogenesis by nitric oxide. Stem Cells International, 2012:895659. Epub 2012 Sep 10.
Carreira BP, Morte MI, Lourenço AS, Santos AI, Inácio Â, Ambrósio AF, Carvalho CM, Araújo IM (2013) Differential contribution of the guanylyl cyclase-cyclic GMP-Protein kinase G pathway to the proliferation of neural stem cells stimulated by nitric oxide. Neurosignals 21:1-13. epub Feb 23, 2012.
Carreira BP, Morte MI, Carvalho CM and Araújo IM (2012) Assessing the influence of neuroinflammation on neurogenesis: in vitro models using neural stem cells and microglia as valuable research tools in Neural Stem Cells and Therapy. Edited by Intech. Editor: Tao Sun. ISBN 979-953-307-213-4
Araújo IM, Carreira BP, Carvalho CM, Carvalho AP (2010) Calpains and delayed calcium deregulation in excitotoxicity. Neurochemical Research 35(12):1966-1969.
Carreira BP, Morte MI, Inácio Â, Rosmaninho-Salgado J, Costa G, Agasse F, Carmo A, Couceiro P, Brundin P, Ambrósio AF, Carvalho CM, Araújo IM (2010) Nitric oxide stimulates the proliferation of neural stem cells bypassing the epidermal growth factor receptor. Stem Cells 28(7):1219-1230.
Rosmaninho-Salgado J, Araújo IM, Álvaro AR, Mendes AF, Ferreira L, Grouzmann E, Mota A, Duarte EP, Cavadas C. (2009) Regulation of catecholamine release and tyrosine hydroxylase in human adrenal chromaffin cells by Interleukin-1β: roles of Neuropeptide Y and nitric oxide. Journal of Neurochemistry 109(3):911-922.
Araújo IM, Gil J, Carreira BP, Mohapel P, Petersén Å, Pinheiro PS, Soulet D, Bahr BA, Brundin P, Carvalho CM (2008) Calpain activation is involved in early caspase-independent neurodegeneration in the hippocampus following status epilepticus. Journal of Neurochemistry 105(3):666-676
Álvaro AR, Martins J, Araújo IM, Rosmaninho-Salgado J, Ambrósio AF, Cavadas C. (2008) Neuropeptide Y stimulates retinal neural cell proliferation – involvement of nitric oxide. Journal of Neurochemistry 105(6):2501-2510.
Yu L, Shen H-Y, Coelho JE, Araújo IM, Huang Q-Y, Day Y-J, Rebola N, Canas PM, Rapp EK, Ferrara J, Taylor D, Müller CE, Linden J, Cunha RA, Chen J-F (2008) A2A receptors modulate motor activity and MPTP neurotoxicity by distinct cellular mechanisms. Annals of Neurology 63(3):338-346.
2016-2018 – Role: Team Member. Research project “Regeneration in the african spiny mouse”. Supported by FCT, PTDC/BIA-ANM/0697/2014, Principal Investigator: Gustavo Tiscornia.
2016 – 2018 – Role: Team Member. Research project “IMPLANTABLE ORGANIC DEVICES FOR ADVANCED THERAPIES (INNOVATE)”. Supported by FCT, PTDC/EEI-AUT/5442/2014, Principal investigator: Henrique Leonel Gomes.
2013 – 2015 – Role: Principal investigator. Research project “Enhancement of endogenous neurogenesis by nitric oxide: identification of S-nitrosylation targets”. Supported by FCT, PTDC/SAU-OSD/0473/2012.
2011-2014 – Role: Principal investigator. Research project “iCALP – Identification of the physiological roles of calpains in the control of cell proliferation and migration in the central nervous system”. Supported by FCT, PTDC/SAU-NMC/112183/2009.
2012-2013 – Role: Principal investigator. Bilateral cooperative project “Identification of the S-nitrosylation targets of NO in the stimulation of endogenous neurogenesis”. Supported by CRUP, Ação Integrada nº E-10/12.
2010-2013 – Role: Team member. PTDC/SAU-NEU/102612/2008.
2007 – 2010 – Role: Team member. PTDC/SAU-NEU/73119/2006.
2007 – 2010 – Role: Team member. PTDC/SAU-NEU/81064/2006.
2013 – present – Delegate of the COST action BM1005 – Gasotransmitters: from basic science to therapeutic applications (ENOG: European Network on Gasotransmitters)
2006 – 2010 – FP6-LIFESCIHEALTH-Ref.19063, Network of European Neuroscience Institutes (ENI-NET), Young Investigator.
Science outreach grants:
2014-2016 – Role: Team member. Outreach project for developing an itinerant exhibition for science centres “”Science Trip – Vamos descascar ciência”. Supported by Ciência Viva (150,000 Euro). Consortium of 5 Science Centres belonging to the Ciência Viva network. Coordination: Centro Ciência Viva Lousal.
2011-2013 – Role: Team member. “Quero mais e melhores células! (Células estaminais: o que são? onde estão? para que servem?)”. Supported by COMPETE – Ciência Viva – 1325.