The Cell Cycle and Cancer Biology Laboratory

alvarotavares
claudiaflorindo

Location

Building 8, Laboratory 1.17

Department of Biomedical Sciences and Medicine.

University of Algarve

Phone: +351 289 244 480

aatavares@ualg.pt

cstavares@ualg.pt

Team Members

Álvaro Tavares  (Principal Investigator)

Claudia Florindo (Independent researcher)

Frederica Sivilva (MSc Student)

Joana Sousa (MSc Student)

About the CCBL

The Cell Cycle and Cancer Biology Laboratory (CCBL) examines the components and regulatory mechanisms of the mitotic machinery, and investigates the mechanisms by which the deregulation of cell division can lead to cancer. The CCBL is focused on two crucial processes during mitosis, chromosome attachment to the spindle (kinetochore function) and the completion of cell division (cytokinesis). Using a broad range of classic genetic and cellular models, the CCBL group has identified a set of new genes that significantly affect chromosome segregation, spindle assembly and abscission. These areas are under active investigation within the CCBL. We further showed that, when mutated, some of these genes cause the formation of tumors in Drosophila, and our main goal is to find if they are equally involved in tumorigenesis in humans.

Claudia Florindo investigates how cells divide. Mainly interested in understanding the fine tuning of the metaphase to anaphase transition and the final event of cytokinesis – abscission. Specifically how the microtubule dynamics affect these processes and what are the key regulators of these events. Ultimately to know how the deregulation of these mechanisms contributes to tumorigenesis. Currently, the CCBL is focused on trying to define the influence of cell polarity over cytokinesis, using CALI (Chromophore-Assisted Laser Inactivation) as the main technique.

Publications (2008-2014)

Silva PM, Reis RM, Bolanos-Garcia VM, Florindo C, Tavares ÁA, Bousbaa H. (2014) Dynein-dependent transport of spindle assembly checkpoint proteins off kinetochores toward spindle poles. FEBS Lett. 588:3265-3273.

Marco A. Campinho, João Saraiva, Claudia Florindo, Deborah M. Power. (2014) “Maternal thyroid hormones are essential for neural development in zebrafish”. Molecular Endocrinology 28:1136-1149

Rafael MS, Laizé V, Florindo C, Ferraresso S, Bargelloni L, Cancela ML. Overexpression of four and a half LIM domains protein 2 promotes epithelial-mesenchymal transition-like phenotype in fish pre-osteoblasts. Biochimie. 2012 May;94(5):1128-34. doi: 10.1016/j.biochi.2012.01.013. Epub 2012 Jan 20.

Pereira H, Barreira L, Mozes A, Florindo C, Polo C, Duarte CV, Custódio L, Varela J. Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae. Biotechnol Biofuels. 2011 Dec 22;4(1):61. doi: 10.1186/1754-6834-4-61. PMID:22192119

Florindo C, Perdigão, J., Fesquet, D., Schiebel, E., Pines, J. and Tavares A.A. (2012) “ Human Mob1 proteins are required for cytokinesis by controlling microtubule stability.” J. Cell Sci 125: 3085-3090.

Tavares A., Gonçalves J., Florindo C, Tavares A.A. and Soares H. (2012) Mob1: defining cell polarity for proper cell division. J. Cell Sci 125:516-527.

Lince-Faria, M., Maffini, S., Orr, B., Ding, Y., Florindo, C., Sunkel, C.E., Tavares, A.A., Johansen, J., Johansen, K. M. and Maiato, H. (2009) “Spatio-temporal control of mitosis by the conserved spindle matrix protein Megator” J. Cell Biology 184:647-657.

Godinho, S. and Tavares, A.A. (2008) “A role for Drosophila Polo protein in chromosome resolution and segregation during mitosis”, Cell Cycle 7:2529 – 2534.

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Team

alvarotavares_team

Projects

EXPL/BEX-BCM/1140/2013 – “Mechanisms of cell polarity in cytokinesis: a Chromophore-Assisted Laser Inactivation approach”, Principal Investigator Cláudia Florindo

Project Leader

PTDC/SAU-OBD/105234/2008- Role of Mob1 and Unc119 proteins in cytokinesis and tumorogenesis. (2010 – 2012)

Colaborator

PTDC/BIA-BCM/69912/2006 (2008 – 2010)

PTDC/SAU-NEU/81153/2006 (2008 – 2010)

Publications (2008-2014)

Silva PM, Reis RM, Bolanos-Garcia VM, Florindo C, Tavares ÁA, Bousbaa H. (2014) Dynein-dependent transport of spindle assembly checkpoint proteins off kinetochores toward spindle poles. FEBS Lett. 588:3265-3273.

Marco A. Campinho, João Saraiva, Claudia Florindo, Deborah M. Power. (2014) “Maternal thyroid hormones are essential for neural development in zebrafish”. Molecular Endocrinology 28:1136-1149

Rafael MS, Laizé V, Florindo C, Ferraresso S, Bargelloni L, Cancela ML. Overexpression of four and a half LIM domains protein 2 promotes epithelial-mesenchymal transition-like phenotype in fish pre-osteoblasts. Biochimie. 2012 May;94(5):1128-34. doi: 10.1016/j.biochi.2012.01.013. Epub 2012 Jan 20.

Pereira H, Barreira L, Mozes A, Florindo C, Polo C, Duarte CV, Custódio L, Varela J. Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae. Biotechnol Biofuels. 2011 Dec 22;4(1):61. doi: 10.1186/1754-6834-4-61. PMID:22192119

Florindo C, Perdigão, J., Fesquet, D., Schiebel, E., Pines, J. and Tavares A.A. (2012) “ Human Mob1 proteins are required for cytokinesis by controlling microtubule stability.” J. Cell Sci 125: 3085-3090.

Tavares A., Gonçalves J., Florindo C, Tavares A.A. and Soares H. (2012) Mob1: defining cell polarity for proper cell division. J. Cell Sci 125:516-527.

Lince-Faria, M., Maffini, S., Orr, B., Ding, Y., Florindo, C., Sunkel, C.E., Tavares, A.A., Johansen, J., Johansen, K. M. and Maiato, H. (2009) “Spatio-temporal control of mitosis by the conserved spindle matrix protein Megator” J. Cell Biology 184:647-657.

Godinho, S. and Tavares, A.A. (2008) “A role for Drosophila Polo protein in chromosome resolution and segregation during mitosis”, Cell Cycle 7:2529 – 2534.

Projects

EXPL/BEX-BCM/1140/2013 – “Mechanisms of cell polarity in cytokinesis: a Chromophore-Assisted Laser Inactivation approach”, Principal Investigator Cláudia Florindo

Project Leader

PTDC/SAU-OBD/105234/2008- Role of Mob1 and Unc119 proteins in cytokinesis and tumorogenesis. (2010 – 2012)

Colaborator

PTDC/BIA-BCM/69912/2006 (2008 – 2010)

PTDC/SAU-NEU/81153/2006 (2008 – 2010)

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