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CBMR researchers discovered a small chemical compound that could potentially be used as anti-cancer and anti-aging drug

A research team led by Wolfgang Link and composed by other CBMR researchers, namely Bibiana Ferreira and Susana Machado, recently published a research article in PLOS ONE, the world’s first multidisciplinary open access journal in the field of science and medicine. The article results from the development of an innovative research in the field of cancer and suggests that LOM612 – a small chemical compound – can be used as a potent FOXO relocator.

The family of FOXO transcription factors is commonly inactivated in human tumours by posttranslational modifications and genetic variation within the FOXO3a gene is consistently associated with human longevity. This scenario represents an attractive therapeutic approach to treat cancer and age-related diseases by pharmacologically activation of FOXO proteins.

In order to identify agents capable of activating FOXOs, Wolfgang Link and his team tested a collection of small chemical compounds using image-based high content screening technology.   The authors were able to show that LOM612 induces nuclear translocation of a FOXO3a reporter protein as well as endogenous FOXO3a and FOXO1 in U2OS cells in a dose-dependent manner. According to the team, this activity does not affect the subcellular localization of other cellular proteins including NFkB or inhibit CRM1-mediated nuclear export. More importantly, LOM612 shows a potent antiproliferative effect in human cancer cell lines.

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Seminar “Uma oportunidade para projetos inovadores na área da saúde e bem-estar”

CBMR is organizing the Seminar “Uma oportunidade para projetos inovadores na área da saúde e bem-estar”, a talk given by José Pereira Leal, scientific director of Healthcare City, an incubator working in the field of health sciences.

The Healthcare City, by Nova Medical School, is the right place for any entrepreneur with an innovative idea and ambition to push forward and grow globally. The goal of Healthcare City is help to form startups and help young entrepreneurs and grow in a global context and expand it worldwide.

Join us, know Healthcare City, present your idea and we could help you to grow!

See more here.

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CBMR represented at the Neptune Evo Devo Symposium 2016

Álvaro Tavares, CBMR researcher, will be one of the invited speakers at the Neptune Evo Devo Symposium 2016. 

The Neptune Evo Devo Symposium is organised by the Neptune Network students, a Marie Curie-funded European project with focus on evo-devo and neurobiology of marine animal models. During four years, the network has gathered students and PIs from nine laboratories across Europe, as well as external partners, in a variety of successful meetings and courses.
This symposium will focus on two important aspects of animal evolution: evolution of the nervous system and evolution of body plans. In line with Neptune’s core interest, emphasis will be put on marine organisms, though not exclusively, and on recent technologies which are becoming increasingly suitable for use in non-model organisms enabling broader comparative studies.
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Hands ON Microscopy Workshop

CBMR Microscopy Unit in collaboration with CCMAR and Izasa Scientific kindly invites you to the next hands on Workshop & demonstrations events.

ON 15Th and 16TH of November

You can bring your own samples
1-    Hands on – Nikon-Vico2

ViCo.2 adopts a novel imaging method to obtain optical sections of microscopic specimens.

Using a conventional fluorescence microscope, structured illumination via an V, W scanning mechanism and processing procedures, equivalent or better results are achieved compared to
those of current, laser based confocal microscopes. Smooth transition between conventional, confocal imaging capabilities permits to optimize resolution versus speed and specimen photo-invasivity.

Spectral and contrast flexibilities are guaranteed by automated multi-band and multi-mode acquisition capabilities. (Up to 6 channels – 2D, 3D, Z and T stack). The system is assemble on a motorized Nikon inverted microscope Ti-E.

Please register on The doodle
(We have limited spots)

http://doodle.com/poll/tu8vccdttx762gu5

 
2-    Hands on  – Andor dsd-2

The Andor Revolution DSD2 is a simple confocal device delivering extra-ordinary imaging performance. Its simplicity lies in a compact patented optical design and laser-free operation, which provide ease of retrofit, flexible fluorophore selection, low maintenance and inherent safety for users. Combining structured illumination and spinning disk technologies with high sensitivity high dynamic range Andor sCMOS cameras, the Revolution DSD2 produces image quality that typically exceeds confocal images captured with laser point scanning systems.

The DSD2 captures images at high frame rates increasing productivity when imaging both fixed and live samples. With three confocal sectioning options, the DSD2 allows the user to trade optical sectioning with signal level and handles a broad magnification range, and sample types from single cell to very thick specimens such as Drosophila embryos and Zebrafish.

The DSD2 can even be used with Macroscopes for larger specimens. This new design can image conventional transmitted light contrast techniques (e.g. phase contrast and DIC) to be combined with the confocal image. The system is assemble on a Nikon stereomicroscopeSMZ25.

Please register on
(We have limited spots)

Please register on The doodle
(We have limited spots)

http://doodle.com/poll/ipr2etnni98774yr

 
You are most welcome to came and join us.
We would like you to bring your own samples.

Please came and enjoy this microscopes and learn how can they help you to solve your scientific questions.
The demos will be on:
Universidade do Algarve – Campus de Gambelas, Ed7 sala 3.37

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CBMR will participate on “1as Jornadas do ABC”

Academic Center will organize on November 24th the “1as Jornadas do ABC”, a meeting to foster collaboration between the Centro Hospitalar do Algarve and CBMR. The meeting, that will be held on Hospital de Faro, aims to promote a set of talks in order to present the research developed on CBMR and the interconnection with Centro Hospitalar do Algarve.

See the program here.

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Sónia Simão won an honorable mention at “Prémio Janssen Inovação 2016”

Sónia Simão, CBMR researcher, won an honorable mention at “Prémio Janssen Inovação 2016” with the project “Aliskiren as a new therapeutic approach for the treatment of diabetic retinopathy”. The project, developed with Daniela F. Santos and Gabriela A. Silva, aims to investigate the RAS (The renin-angiotensin system) in diabetic conditions and test the effectiveness of Aliskiren, as a drug with potential to be used in the design of new approaches for the treatment of diabetic retinopathy.

More about the project: Unhealthy lifestyles and an aging population significantly contribute to the high incidence of diabetes worldwide. This metabolic disease culminates in threatening complications such as diabetic retinopathy (DR). This visual disorder is the main cause of acquired blindness among working age adults. An effective therapy for DR has not been established so far; current therapies halt but do not revert the disease and encompass some adverse side effects. Therefore, further research is needed to better understand the disease and define new therapeutic targets.
The renin-angiotensin system (RAS) has been identified in the eye and might contribute to ocular damage. The present study was designed to investigate the RAS in diabetic conditions. For this we will focus on the retinal pigment epithelium (RPE), a retinal cell type responsible for maintaining the integrity of the retina and known to be deeply compromised in DR.
The direct renin inhibitors are a new generation of RAS inhibitors and aliskiren is the only in this class approved for medical use. Based on its action, the present study tested the efficacy of this drug in decreasing downstream events derived from RAS activation such as oxidative stress and angiogenesis.
We observed that RPE cells express the main components of the RAS and this system was significantly deregulated by hyperglycemia. Exposure of RPE cells to aliskiren inhibited the activity of renin and consequently decreased the levels of angiotensin II. Aliskiren also reduced the translocation of the (pro)renin receptor to the cellular membrane of RPE cells preventing the activation of ERK1/2. Furthermore, RAS activation induced oxidative stress and angiogenesis in RPE cells, which were effectively prevented by aliskiren.
In conclusion, our findings demonstrate that aliskiren successfully inhibit the RAS in RPE cells. We described in detail the mode of action of aliskiren in the RPE cells and found this drug to have antioxidant and anti-angiogenic properties. These new features of aliskiren suggest that this drug has potential to be used in the design of new approaches for the treatment of diabetic retinopathy.

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Ana Teresa Maia investigate genetic alteration “made in Portugal”

Ana Teresa Maia, CBMR researcher, is participating in a pioneering study about a genetic alteration that seems to be born in Portugal, approximately two thousand years ago. This genetic alteration, responsible for the increased risk of hereditary cancer (especially breast cancer), is a mutation that researchers believe that could have been carried (by sailors, traders and emigrants) from Portugal to other parts of the world.

Note that, as Ana Teresa Maia states: “this mutation of the genes have been already identified in French and Australian families with Portuguese ancestors”.

So, the main goal of researchers seems to be understand how malignant is that mutation and how was developed in Portugal. In order to achieve that, researchers and doctors will work together, with the help of two associations – the Portuguese Association for Cancer Research and the Portuguese Association to support people with alterations in genes related to hereditary cancer.

The project, that ensures to bring unreleased revelations to the cancer field, have a solid foundation on CBMR research and specially on the research of Ana Teresa Maia on gene expression.

See more here: News – Jornal de Notícias 

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CBMR researcher share knowledge in microscopy around the country

Cláudia Florindo, CBMR researcher, will be one of the teachers of  the “II Quantitative Fluorescence Microscopy Course”, that will be held from 28th November to 2nd December, at Microscopy Imaging Center of Coimbra.

This one-week intensive microscopy course aims to give to the participants a theoretical understanding of state-of-the-art equipment used in quantitative fluorescence microscopy, as well as an hands-on experience with: wide-field microscopy, laser scanning and spinning disk confocal microscopy, deconvolution methods, multi-photon microscopy, and digital image processing and analysis.

The goal of the course is to provide attendees with the knowledge and expertise to implement cutting-edge microscopic methods within their own laboratories.

See more information here.

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Ramiro Magno represent CBMR at the 11th Meeting of the Spanish Society for Developmental Biology

Ramiro Magno, CBMR researcher, will be participating in the 11th Meeting of the Spanish Society for Developmental Biology, in Girona, from 19th to 22th october, with the poster “STAGER, staging tool for the analysis of expression results”.

The meeting aims to be an excellent opportunity to discover the latest experimental approaches and future directions in Developmental and Cell Biology as well as to discuss new data and ideas. The program includes world leaders in different fields and a prominent place is also reserved for posters and oral contributions that will be selected from the abstracts for all sessions. The 3-day meeting will include three Pre-meeting workshops on: Genome editing, Single cell Omics and Cell dynamics, and over 10 sessions, with broad discussion on the following topics:

  • Modelling Cell Behaviour and morphogenesis
  • Evo-Devo & Genomics
  • Biological Oscillators
  • Signalling in Development & disease
  • Cell Biology
  • Neural Development
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Inês Araújo will represent CBMR on ORBEA

Inês Araújo, CBMR researcher and head of the Animal Facility Unit, will represent the centre on “Simpósio Nacional Órgãos Responsáveis pelo bem-estar dos animais usados em investigação científica” – ORBEA, that will be held on  18th october on University of Oporto. The event, the first of this kind held in Portugal, aims to bring together all the bodies responsible for animal welfare, from various research institutions, and work on the standardization of practices and principles throughout the country.